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Department of Biochemistry, Microbiology and Immunology Wei Xiao

Selected Honours and Awards

  • University of Saskatchewan Distinguished Researcher of the Year Award (2008)
  • College of Medicine Graduate Student Society (CMGSS), U. of Sask. Supervisor of the Year Award (2007)
  • Biographee, CANADA at the Millennium (2000)
  • Biographee, Canadian Who’s Who (since 1996)

Education

  • B.Sc. (1982) Nanjing Agricultural University
  • M.Sc. (1984) University of Toronto
  • Ph.D. (1988) University of Saskatchewan
  • PDF (1990-1992) Harvard University

Research Interests

Click here for a list of ongoing research projects.

Ubiquitin (Ub) is an abundant, ubiquitous and highly-conserved small protein (76 amino acids) found in all eukaryotic cells, from unicellular yeasts to human. Through a series of enzymatic reactions, Ub is attached to the target protein with the help of an Ub conjugating enzyme (E2 or Ubc) and an Ub ligase (E3), followed by the formation of a multimeric Ub chain known as the poly-Ub chain. Target proteins attached by poly-Ub are sent for degradation by the 26S proteosome, and this process serves as an important means of regulation involved in numerous cellular activities.  Its importance is appreciated by the 2004 Nobel Prize award to the discovery of ubiquitination and its involvement in the target protein degradation.  Careful investigation reveals that the above Ub chain is formed via a surface Lys48 residue of an incoming Ub attached to the C-terminal Gly residue. It was subsequently found that the Ub chain can also be formed via a surface Lys63 residue.  Among over a dozen of Ubcs found in any organisms, only one, Ubc13, is capable of linking Ub through Lys63. The unique feature of Ubc13 is due to its binding to another protein known as Uev (Ubc enzyme variant), which is also absolutely required for the process. More importantly, proteins attached by Lys63-linked Ub are not targeted for degradation, but for altering activities. Furthermore, among other types of ubiquitination, mono-ubiquitination is also believed to be a novel regulatory mechanism of the target protein activity, which expands the horizon of ubiquitination functions. My laboratory is primarily interested in the discovery of above non-conventional ubiquitinaiton processes and in defining the molecular mechanisms of the related pathways. Since a few well characterized non-conventional ubiquitination target proteins are involved in cellular metabolisms such as DNA damage tolerance, cell cycle checkpoint, innate immunity and stress response, and that these processes play critical roles in human diseases like cancer, this study will have direct impacts on the diagnosis and treatment of diseases including cancer.

Selected Publications

Click here for the full list of publications.

Du, Y., Ma, C., Moore, S.A. and Xiao, W. (2024) Zinc-finger 4 negatively controls the transcriptional activator Fzf1 in Saccharomyces cerevisiae. mLife 3: 391-402. [Text]

Rybchuk, J. and Xiao, W. (2024) Dual activities of a silencing information regulator complex in yeast transcriptional regulation and DNA-damage response. mLife 3: 207-218.[Text]

Venkadakrishnan, J., Lahane, G., Dhar, A., Xiao, W., Bhat, K.M., Pandita, T.K. and Bhat, A. (2023) Implications of translesion DNA synthesis polymerases on genomic stability and human health. Mol. Cell. Biol. 43: 401-425. [Text]

Hu, R., Li, X., Hu, Y., Zhang, R., Lv, Q., Zhang, M., Sheng, X., Zhao, F., Chen, Z., Ding, Y., Yuan, H., Wu, X., Xing, S., Yan, X., Bao, F., Wan, P., Xiao, L., Wang, X., Xiao, W., Decker, E.L., van Gessel, N., Renault, H., Wiedemann, G., Horst, N.A., Haas, F.B., Wihelmsson, K.I., Ullrich, K.K., Neumann, E., Lv, B., Liang, C., Du, H., Lu, H., Gao, Q., Cheng, Z., You, H., Xin, P., Chu, J., Huang, C.-H., Liu, Y., Dong, S., Zhang, L., Chen, F., Deng, L., Duan, F., Zhao, W., Li, K., Li, Z., Li, X., Cui, H., Zhang, Y., Ma, C., Zhu, R., Jia, Y., Wang, M., Hasebe, M., Fu, J., Goffinet, B., Ma, H., Rensing, S.A., Reski, R. and He. Y. (2023) Adaptive evolution of the enigmatic Takakia now facing climate change in Tibet. Cell186: 3558-3576. [Text]

Fan, L., Zhang, W., Rybchuk, J., Luo, Y. and Xiao, W. (2023) Genetic dissection of budding yeast PCNA mutations responsible for the regulated recruitment of Srs2 helicase. mBio 14: 00315-23. [Text]

Lin, A., Chumala, P., Du, Y., Ma, C., Wei, T., Xu, X., Luo, Y., Katselis, G.S. and Xiao, W. (2023) Transcriptional activation of budding yeast DDI2/3 through chemical modifications of Fzf1. Cell Biol. Toxicol. 39: 1531-1547. [Text]

Kumasaruge, I., Wen, R., Wang, L., Gao, P., Peng, G. and Xiao, W. (2023) Systematic characterization of Brassica napus UBC13 genes involved in DNA-damage response and K63-linked polyubiquitination. BMC Plant Biol. 23: 24. [Text]

Wang, L., Yang. K., Wang, Q. and Xiao, W. (2023) Genetic analysis of DNA-damage tolerance pathways in Arabidopsis. Plant Cell Rep. 42: 153-164. [Text]

Luo, R., Yang, K. and Xiao, W. (2023) Plant deubiquitinases: from structure and activity to biological functions. Plant Cell Rep. 42: 469-486. [Text]

lv, , Han, S., Wang, L., Xia, J., Li, P., Hu, R., Wang, J., Gao, L., Chen, Y., Wang, Y., Du, J., Bao, F., Hu, Y., Xu, X., Xiao, W. and He, Y. (2022) TEB/POLQplays dual roles in protecting Arabidopsis from NO-induced DNA damage. Nucleic Acids Res. 50: 6820-6836. [Text]
Yang, K. and Xiao, W. (2022) Functions and mechanisms of the Ubc13-UEV complex and Lysine 63-linked polyubiquitination in plants. J. Exp. Bot. 73: 5372-5387. [Text]
Li, X., Zhao, R., Li, S., Wang, Y., Wang, X., Yang, W., Yang, M., Xiao, W., Yang, S., Lin, X., Zheng, X., Ma, X., Zhao, L., Xiao, W. and Cao, L. (2022) Global reprogramming of xylose metabolism in Saccharomyces cerevisiae efficiently produces ethanol from lignocellulose hydrolysates. Crop. Produc. 179: 114666. [Text]
Xin, Y., Wang, J., Wu, Y., Li, Q., Dong, M., Liu, C., He, Q., Wang, R., Wang, D., Jiang, S., Xiao, W., Tian, Y. and Zhang, W. (2022) Identification of Nanog as a novel inhibitor of Rad51. Cell Death & Disease 13: 193. [Text]
Sun, Y., Kong, M., Li, X., Li, Q., Xue, Q., Hou, J., Jia, Z., Lei, Z., Xiao, W., Shi, S. and Cao, L. (2022) Metabolic and evolutionary engineering of diploid yeast for the production of first- and second-generation ethanol. Bioeng. Biotechnol. 9: 835928. [Text]
Zeng, C. and Xiao, W. (2022) Molecular cloning and functional characterization of UBC13 and MMS2 from Candida albicans. Gene 816: 146163. [Text]

Bi, T., Niu, X., Qin, C. and Xiao, W. (2021) Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells. Rep. 11: 21364. [Text]

Kong, M., Li, X., Li, T., Zhao, X., Jin, M., Zhou, X., Gu, H., Mrsa, V., Xiao, W. and Cao, L. (2021) OverexpressingCCW12 in Saccharomyces cerevisiae enables highly efficient ethanol production from lignocellulose hydrolysates. Bioresource Technol. 337: 125487. [Text]

Liu, L., Jin, , Huang, M., Zhu, Y., Yuan, W., Kang, Y., Kong, M., Alim S., Jia, Z., Xu, Z., Xiao, W. and Cao, L. (2021) Engineered polyploid yeast strains enable efficient xylose utilization and ethanol production in corn hydrolysates. Front. Bioeng. Biotechnol. 9: 655272. [Text]
Zhu, Y., Zhang, J., Zhu, L., Jia, Z., Li, Q., Xiao, W. and Cao, L. (2021) Minimize the xylitol production in Saccharomyces cerevisiae by balancing the xylose redox metabolic pathway. Bioeng. Biotechnol. 9: 639595. [Text]

Fan, L., Bi, T., Wang, L. and Xiao, W. (2020) DNA-damage tolerance through PCNA ubiquitination and sumoylation. Bioch J. 477: 2655-2677. [Text]

Bai, Z., Wei, M., Li, Z. and Xiao, W. (2020) Drosophila Uev1a is dually required for Ben-dependent DNA-damage response and fly mobility. Cell. Signal. 74: 109719. [Text]

Wu, Z., Andersen, P.L., Moraes, T., McKenna, S.A., Zhang, Y., Zhang, W., Ellison, M.J. and Xiao, W. (2020) Uev1A amino terminus stimulates poly-ubiquitin chain assembly and is required for NF-кB activation. Cell. Signal. 74: 109712. [Text]

Zhu, L., Li, P., Sun, T., Kong, M., Li, X., Ali, S., Liu, W., Fan, S., Qiao, J., Li, S., Peng, L., He, B., Jin, M., Xiao, W. and Cao, L. (2020) Overexpression of SFA1 in engineered Saccharomyces cerevisiae to increase xylose utilization and ethanol production from different lignocellulose hydrolysates. Bioresource Technol 313: 123724. [Text]

Yang, D., Sun, Y., Chen, J., Zhang, Y., Fan, S., Huang, M., Xie, X., Cai, Y., Shang, Y., Gui, T., Sun, L., Hu, J., Dong, J., Yeap, L.-S., Wang, X., Xiao, W. and Meng, F. (2020) REV7 is required for processing AID initiated DNA lesions in activated B cells. Nat. Commun. 11: 2812. [Text]

Wang, F., Li, P., Shao, Y., Li, Y., Zhang, K., Li, M., Wang, R., Zheng, S., Wang, Y., Song, S., Liu, F., Xiao, W. and Li, X. (2020) Site-specific proteolytic cleavage prevents ubiquitination and degradation of human REV3L, the catalytic subunit of DNA polymerase ζ. Nucleic Acids Res. 48: 3619-3637. [Text]

Zang, Y., Gong, Y., Wang, Q., Guo, H. and Xiao, W. (2020) Arabidopsis OTU1, a linkage-specific deubiquitinase, is required for ER-associated protein degradation. Plant J. 101: 141-155. [Text]

Niu, X., Chen, W., Bi, T., Lu M., Qin, Z. and Xiao, W. (2019) Rev1 plays central roles in mammalian DNA-damage tolerance in response to UV irradiation. FEBS J. 286: 2711-2725. [Text]

Li, J., Jia, Y., Lin, A., Hanna, M., Chelico, L., Xiao, W. and Moore, S. (2019) Structure of yeast Ddi2, a highly inducible detoxifying metalloenzyme from S. cerevisiae. J. Biol. Chem. 294: 10674-10685. [Text]

Wang, L., Wen, R., Wang, J., Xiang, D., Wang, Q., Zang, Y., Wang, Z., Huang, S., Li, X., Datla, R., Fobert, P.R., Wang, H., Wei, Y. and Xiao, W. (2019) Arabidopsis UBC13 differentially regulates two programmed cell death pathways in responses to pathogen and low temperature stress. New Phytologist 221: 919-934. [Text]

Lin, A., Zeng, C., Wang, Q., Zhang, W., Li, M., Hanna, M. and Xiao, W. (2018) Utilization of a strongly-inducible DDI2 promoter to control gene expression in Saccharomyces cerevisiae. Front. Microbiol. 9: 2736. [Text]

Rout, M.K., Lee, B.L., Lin, A., Xiao, W. and Spyracopoulos, L. (2018) Active site gate dynamics modulate the catalytic activity of the ubiquitination enzyme E2-25K. Sci. Rep. 8: 7002. [Text]

Wu, Z., Neufeld, H., Torlakovic, E. and Xiao, W. (2018) Uev1A-Ubc13 promotes colorectal cancer metastasis through regulating CXCL1 expression via NF-кB activation. Oncotarget 9: 15952-15967. [Text]

Li, Z., Wang, Y., Li, Y., Zhang, Y., Zhang, Z., Ren, X., Yin, W., Wang, G., Zhu, B., Xiao, W. and Zhang, W. (2018) Ube2s stabilizes β-Catenin through K11-linked polyubiquitination to promote mesendoderm specification and colorectal cancer development. Cell Death & Disease 9: 456. [Text]

Zhao, H., Wang, Q., Liu, C., Shang, Y., Wen, F., Wang, F., Liu, W., Xiao, W. and Li, W. (2018) A role for respiration in regulating meiosis initiation in Saccharomyces cerevisiae. Genetics 208: 1181-1194. [Text]

Qin, Z., Jiang, W., Wang, G., Sun, Y. and Xiao, W. (2018) Linear ubiquitin chain induces apoptosis and inhibits tumor growth. Apoptosis 23: 16-26. [Text]

Zhang, Y., Li, Y., Yang, X., Wang, J., Wang, R., Qian, X., Zhang, W. and Xiao, W. (2018) Uev1A-Ubc13 catalyzes K63-linked ubiquitination of RHBDF2 to promote TACE maturation. Cell. Signal. 42: 155-164. [Text]

Niu, C., Wang, D., Liu, X., Liu, H., Liu, X., Feng, E., Pan, C., Wang, R., Xiao, W., Liu, X., Liu, X., Zhu, L. and Wang, H. (2017) An H-NS family protein, Sfh, regulates acid resistance by inhibition of glutamate decarboxylase expression in Shigella flexneri 2457T. Front. Microbiol. 8: 1923. [Text]

Zhang, W., Zhuang, Y., Zhang, Y., Yang, X., Zhang, H., Wang, G., Yin, W., Wang, R., Zhang, Z. and Xiao, W. (2017) Uev1A facilitates osteosarcoma differentiation by promoting Smurf1-mediated Smad1 ubiquitination and degradation. Cell Death & Diseases 8: e2974. [Text]

Bhat, A., Qin, Z., Wang, G., Chen, W. and Xiao, W. (2017) Rev7, the regulatory subunit of Polζ, undergoes UV-induced and Cul4-dependent degradation. FEBS J. 284: 1790-1803. [Text]

Niu, C., Yang, J., Liu, H., Cui, Y., Xu, H., Wang, R., Liu, X., Feng, E., Wang, D., Pan, C., Xiao, W., Liu, X., Zhu, L. and Wang, H. (2017) Role of the virulence plasmid in acid resistance of Shigella flexneri. Sci. Rep. 7: 46465. [Text]

Qin, Z., Bai, Z., Sun, Y., Niu, X. and Xiao, W. (2016) PCNA-Ub polyubiquitination inhibits cell proliferation and induces cell-cycle checkpoints. Cell Cycle 15: 3390-3401. [Text]

Fan, L. and Xiao, W. (2016) The Pol30-K196 residue plays a critical role in budding yeast DNA postreplication repair through interaction with Rad18. DNA Repair 47: 42-48. [Text]

Guo, H., Wen, R., Wang, Q., Datla, R. and Xiao, W. (2016) Three Brachypodium distachyon Uev1s promote Ubc13-mediated Lys63-linked polyubiquitination and confer different functions. Front. Plant Sci. 7: 1551. [Text]

Yuan, S., Zhang, Z., Zheng, C., Zhao, Z., Wang, Y., Feng, L., Niu, G., Wang, C., Wang, J., Feng, H., Bao, F., Hu, Y., Cao, Y., Ma, L., Wang, H., Kong, D., Xiao, W., Lin, H. and He, Y. (2016) Arabidopsis cryptochrome 1 functions in nitrogen regulation of flowering. Proc. Natl. Acad. Sci. USA 113: 7661-7666. [Text]

Tan, S., Liu, F., Pan, X., Zang, Y., Jin, F., Zu, W., Q, X., Xiao, W. and Yin, L. (2016) CSN6, a subunit of the COP9 signalosome, is involved in early response to iron deficiency in Oryza sativa. Sci. Rep. 6: 25485. [Text]

Xu, X., Lin, A., Zhou, C., Blackwell, S., Zhang, Y., Wang, Z., Feng, Q., Guan, R., Hanna, M.D., Chen, Z. and Xiao, W. (2016) Involvement of budding yeast Rad5 in translesion DNA synthesis through physical interaction with Rev1. Nucleic Acids Res. 44: 5231-5245. [Text]

Wang, J., Zhang, Y., Hou, J., Qian, X., Zhang, H., Zhang, Z., Li, M., Wang, R., Liao, K., Wang, Y., Li, Z., Zhong, D., Wan, P., Dong, L., Liu, F., Wang, X., Wan, Y., Xiao, W. and Zhang, W. (2016) Ube2s regulates Sox2 stability and mouse ES cell maintenance. Cell Death Differ. 23: 393-404. [Text]

Guo, H., Wen, R., Liu, Z., Datla, R. and Xiao, W. (2016) Molecular cloning and functional characterization of two Brachypodium distachyon UBC13 genes whose products promote K63-linked polyubiquitination. Front. Plant Sci. 6:1222. [Text]

Bhat, A., Wu, Z., Maher, V.M., McCormick, J.J. and Xiao, W. (2015) Rev7/Mad2B plays a critical role in the assembly of a functional mitotic spindle. Cell Cycle 14: 3929-3938. [Text]

Li, J., Biss, M., Fu, Y., Xu, X., Moore, S. and Xiao, W. (2015) Two duplicated genes DDI2 and DDI3 in budding yeast encode a cyanamide hydratase and are induced by cyanamide. J. Biol. Chem. 290: 12664-12675. [Text]

Xu, X., Blackwell, S., Lin, A., Li, F., Qin, Z. and Xiao, W. (2015) Error-free DNA-damage tolerance in Saccharomyces cerevisiae. Mutat. Res. - Rev. 764: 43-50. [Text]

Xue, C., Liang, K., Liu, Z., Wen, R. and Xiao, W. (2015) Similarities and differences between Arabidopsis PCNA1 and PCNA2 in complementing the yeast DNA damage tolerance defect. DNA Repair 28: 28-36. [Text]

Rout, M.K., Hodge, C.D., Markin, C.J., Xu, X., Glover, J.N.M., Xiao, W. and Spyracopoulos, L. (2014) Stochastic gate dynamics regulate the catalytic activity of ubiquitination enzymes. J. Am. Chem. Soc. 136: 17446-17458. [Text]

Wen, R., Wang, S., Xiang, D., Venglat, P., Shi, X., Zang, Y., Datla, R., Xiao, W. and Wang, H. (2014) UBC13, an E2 enzyme for Lys63-linked ubiquitination, functions in root development by affecting auxin signaling and Aux/IAA protein stability. Plant J.  80: 424-436. [Text]

Wu, Z., Shen, S., Zhang, Z., Zhang, W. and Xiao, W. (2014) Ubiquitin-conjugating enzyme complex Uev1A-Ubc13 promotes breast cancer metastasis through nuclear factor-κB mediated matrix metalloproteinase-1 gene regulation. Breast Cancer Res. 16: R75. [Text]

Cao, L., Tang, X., Zhang, X., Zhang, J., Tian, X., Wang, J., Xiong, M. and Xiao, W. (2014) Two-stage transcriptional reprogramming in Saccharomyces cerevisiae for optimizing ethanol production from xylose. Metab. Eng. 24: 150-159. [Text]

Ball, L.G., Xu, X., Blackwell, S., Hanna, M.D., Lambrecht, A.D. and Xiao, W. (2014) The Rad5 helicase activity is dispensable for error-free DNA post-replication repair. DNA Repair 16: 74-83. [Text]

Qin, Z., Lu, M., Xu, X., Hanna, M., Shiomi, N. and Xiao, W. (2013) DNA-damage tolerance mediated by PCNA•Ub fusions in human cells is dependent on Rev1 but not Polη. Nucleic Acids Res. 41: 7356-7369. [Text]

Bhat, A., Andersen, P.L., Qin, Z. and Xiao, W. (2013) The Rev3 subunit of Polζis required for maintaining fragile site stability in human cells. Nucleic Acids Res. 41: 2328-2339. [Text]

Wei, T., Zhang, C., Xu, X., Hanna, M., Zhang, X., Wang, Y., Dai, H. and Xiao, W. (2013) Construction and evaluation of two biosensors based on yeast transcriptional response to genotoxic chemicals. Biosensors and Bioelectronics. 44: 138-145. [Text]

Wen, R., Li, J., Xu, X., Cui, Z. and Xiao, W. (2012) Zebrafish Mms2 promotes K63-linked polyubiquitination and is involved in p53-mediated DNA-damage response. DNA Repair 11: 157-166. [Text]

Xiang, D., Yang, H., Venglat, P., Cao, Y., Wen, R., Ren, M., Stone, S., Wang, E., Wang, H., Xiao, W., Weijers, D., Berleth, T., Laux, T., Selvaraj, G., Datla, R. (2011) POPCORN functions in the auxin pathway to regulate embryonic body plan and meristem organization in Arabidopsis. Plant Cell 23: 4348-4367. [Text]

Andersen, P.L., Xu, F., Ziola, B., McGregor, W.G. and Xiao, W. (2011) Sequential assembly of translesion DNA polymerases at UV-induced DNA damage sites. Mol. Biol. Cell 22: 2373-2383.  [Text]

Wang, S., Wen, R., Shi, X., Lambrecht, A., Wang. H. and Xiao, W. (2011) RAD5A and REV3 constitute two alternative mechanisms of DNA damage tolerance in Arabidopsis. DNA Repair 10: 620-628. [Text]

Zhang, M., Zhang, C., Li, J., Hanna, M., Zhang, X., Dai, H. and Xiao, W. (2011) Inactivation of YAP1 enhances sensitivity of the yeast RNR3-lacZ genotoxicity testing system to a broad range of DNA-damaging agents. Toxicol. Sci. 120: 310-321. [Text]

Markin, C.J., Saltibus, L., Kean, M., McKay, R., Xiao, W. and Spyracopoulos, L. (2010) Catalytic proficiency of ubiquitin conjugation enzymes: balancing pKa suppression, entropy, and electrostatics. J. Am. Chem. Soc. 132: 17775-17786. [Text]

Pastushok, L., Hanna, M. and Xiao, W. (2010) Constitutive fusion of ubiquitin to PCNA provides DNA damage tolerance independent of translesion polymerase activities. Nucleic Acids Res. 38: 5047-5058. [Text]

Markin, C.J., Xiao, W. and Spyracopoulos, L. (2010) Mechanism for recognition of polyubiquitin chains: balancing affinity through interplay between multivalent binding and dynamics.  J. Am. Chem. Soc. 132: 11247-11258. [Text]

Zhang, M., Hanna, M., Li, J., Butcher, S., Dai, H. and Xiao, W. (2010) Creation of a hyperpermeable yeast strain to genotoxic agents through combined inactivation of PDR and CWP genes. Toxicol. Sci. 113: 401-411. [Text]

Ball, L.G., Zhang, K., Cobb, J.A., Boone, C. and Xiao, W. (2009) The yeast Shu complex couples error-free PRR to homologous recombination. Mol. Microbiol. 73: 89-102. [Text]

Fu, Y., Pastushok, L. and Xiao, W. (2008) DNA damage-induced gene expression in Saccharomyces cerevisiae. FEMS Microbol. Rev. 32: 908-926. [Text]

Huen, M.S.Y., Yuan, J., Yamamoto, M., Akira, S., Ashley, C., Xiao, W. and Chen, J. (2008) Noncanonical E2 variant-independent function of UBC13 in promoting checkpoint protein assembly. Mol. Cell. Biol. 19: 6104-6112. [Text]

Anderson, H.J., Vonarx, E.J., Pastushok, L., Nakagawa, M., Katafuchi, A., Gruz, P., Di Rubbo, A., Grice, D.M., Osmond, M.J., Sakamoto, A., Nohmi, T., Xiao, W. and Kunz, B.A. (2008) Arabidopsis thaliana Y-family DNA polymerase η catalyses translesion synthesis and interacts functionally with PCNA2. Plant J. 55: 895-908. [Text]

Fu, Y., Zhu, Y. Zhang, K., Yeung, M., Durocher, D. and Xiao, W. (2008) Rad6-Rad18 mediates a eukaryotic SOS response by ubiquitinating the 9-1-1 checkpoint clamp. Cell 133: 601-611. [Text]

Zhang, M., Liang, Y., Zhang, X., Xu, Y., Dai, H. and Xiao, W. (2008) Deletion of yeast CWP genes enhances cell permeability to genotoxic agents. Toxicol. Sci. 103: 68-76. [Text]

Wen, R., Torres-Acosta, J.A., Pastushok, L., Lai, X., Pelzer, L., Wang, H. and Xiao, W. (2008) Arabidopsis UEV1D promotes lysine-63-linked polyubiquitination and is involved in DNA damage response. Plant Cell 20: 213-227. [Text]

Andersen, P.L., Xu, F. and Xiao, W. (2008) Eukaryotic DNA damage tolerance and translesion synthesis through covalent modifications of PCNA. Cell Res. 18: 162-173. [Text]

Barbour, L., Ball, L.G., Zhang, K. and Xiao, W. (2006) DNA damage checkpoints are involved in postreplication repair. Genetics 174: 1789-1800. [Text]

Syed, N.A., Andersen, P.L., Warrington, R.C. and Xiao, W. (2006) Uev1A, a ubiquitin conjugating enzyme variant, inhibits stress-induced apoptosis through NF-κB activation. Apoptosis 11: 2147-2157. [Text]

Fu, Y. and Xiao, W. (2006) Identification and characterization of CRT10 as a novel regulator of Saccharomyces cerevisiae ribonucleotide reductase genes. Nucleic Acids Res. 34: 1876-1883. [Text]

Barbour, L. and Xiao, W. (2006) Mating type regulation of cellular tolerance to DNA damage is specific to the DNA postreplication repair and mutagenesis pathway. Mol. Microbiol. 59: 637-650. [Text]

Andersen, P., Zhou, H., Pastushok, L., Moraes, T., McKenna, S., Ziola, B., Ellison, M.J., Dixit, V.M. and Xiao, W. (2005) Distinct regulation of Ubc13 functions by two Uev proteins Mms2 and Uev1A. J. Cell Biol. 170: 745-755. [Text]

Pastushok, L., Moraes, T.F., Ellison, M.J. and Xiao, W. (2005) A single Mms2 "key" residue insertion into a Ubc13 pocket determines the interface specificity of a human Lys63 ubiquitin conjugation complex. J. Biol. Chem. 280: 17891-17900. [Text]

Zhu, Y and Xiao, W. (2004) Pdr3 is required for DNA damage induction of MAG1 and DDI1 via a bi-directional promoter element. Nucleic Acids Res. 32: 5066-5075. [Text]

Zhou, H., Wertz, I., O'Rourke, K., Ultsch, M., Seshagiri, S., Eby, M., Xiao, W. and Dixit, V.M. (2004) Bcl10 activates the NF-κB pathway through ubiquitination of NEMO. Nature 427: 167-171. [Text]

Hanna, M.D., Meadows, K.L., Chow, B.L., Jinks-Robertson, S., Doetsch, P.W. and Xiao, W. (2004) Involvement of two endonuclease III homologs in the base excision repair for the processing of DNA alkylation damage in Saccharomyces cerevisiae. DNA Repair 3: 51-59 [Text]

Fu, Y. and Xiao, W. (2003) Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization. DNA Repair 2: 1435-1447 [Text]

Jia, X. and Xiao, W. (2003) Compromised DNA repair enhances sensitivity of the RNR3-lacZ genotoxic testing system. Toxicol. Sci. 75: 82-88. [Text]

McKenna, S., Moraes, T., Pastushok, L., Ptak, C., Xiao, W., Spyracopoulos, L. and Ellison, M.J. (2003) An NMR based model of the ubiquitin-bound human ubiquitin conjugation complex Mms2/Ubc13: The structural basis for lysine 63 chain catalysis. J. Biol. Chem. 278: 13151-13158. [Text]

Brown, M., Zhu, Y., Hemmingsen, S, and Xiao, W. (2002) Structural and functional conservation of error-free postreplication repair in Schizosaccharomyces pombe. DNA Repair 1: 869-880. [Text]

Li, Z., Xiao, W., McCormick, J.J. and Maher, V.M. (2002) Identification of a protein essential for a major pathway used by human cells to avoid UV-induced DNA damage. Proc. Natl. Acad. Sci. USA 99: 4459-4464. [Text]

Broomfield, S. and Xiao, W. (2002) Suppression of genetic defects within the RAD6 pathway by srs2 is specific for error-free postreplication repair but not for damage induced mutagenesis. Nucleic Acids Res. 30: 732-739. [Text]

McKenna, S., Spyracopoulos, L., Moraes, T., Pastushok, L., Ptak, C., Xiao, W. and Ellison, M.J. (2001) Non-covalent interaction between ubiquitin and the human DNA repair protein Mms2 is required for Ubc13-mediated poly-ubiquitination. J. Biol. Chem. 276: 40120-40126. [Text]

Moraes, T.F., Edwards, R.A., McKenna, S., Pastushok, L., Xiao, W., Glover, J.N.M. and Ellison, M.J. (2001) Crystal structure of the human ubiquitin conjugating enzyme complex, hMms2-hUbc13. Nature Structural Biol. 8: 669-673. [Text]

Xiao, W., Chow, B.L., Broomfield, S. and Hanna, M. (2000) The Saccharomyces cerevisiae RAD6 group is composed of an error-prone and two error-free postreplication repair pathways. Genetics 155: 1633-1641. [Text]

Chamankhah, M., Fontanie, T. and Xiao, W. (2000) The Saccharomycescerevisiae mre11(ts) allele confers a separation of DNA repair and telomere maintenance functions. Genetics 155: 569-576. [Text]